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. 2016 Jul 26;6:24530. doi: 10.1038/srep24530

Figure 4. DENV NS1 glycoprotein induced IFN-λ1 production through activation of NF-κB signaling.

Figure 4

A549 cells infected by mock or DENV or transfected with a plasmid encoding NS1 or NS4B gene, as indicated, were collected. NF-κB DNA-binding activity was determined in nuclear extracts, as described in Methods (A). Nuclear extracts pre-incubated with wild-type or mutant NF-κB oligonucleotides served as controls. (B) shows analysis from at least three independent experiments. After transfection of a plasmid encoding NS1, A549 cells were treated with different doses of the NF-κB inhibitor Bay11-7082 (C) or PDTC (D), and the expression of IFN-λ1 mRNA and IFN-λ1 protein was determined by RT/PCR and ELISA, respectively. An approach similar to (C) was conducted, but DCs were studied instead (E). Cpt, competitors; Wt, wild-type; Mt, mutant. The analysis was performed by ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. Ctl, control.