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. 2016 Jul 26;6:24530. doi: 10.1038/srep24530

Figure 5. DENV-induced and NS1-induced IFN-λ1 expression was IRF-3-dependent.

Figure 5

A549 cells were infected by mock or DENV for different time periods, and the expression of total or phosphorylated IRF-3 and -7 was determined by Western blotting (A). Expression of total or phosphorylated IRF-3 in A549 cells transfected with plasmids encoding NS1 or NS4B was determined (B). The siRNA interference approach with three sets of duplexes successfully knocked down expression of IRF-3, which reduced NS1-induced IFN-λ1 mRNA expression and protein production (C). Similar to the experiments shown in (A,C), DENV-induced IRF-3 activation and the effect of IRF-3 knockdown were determined in human DCs (D,E, respectively). The data show representative results and analysis pooled from at least three independent experiments examining different donor DCs. The analysis was performed by ANOVA, as described in the Methods. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.