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. 2016 Jul 26;6:24530. doi: 10.1038/srep24530

Figure 6. Defective migration of DENV-infected DCs with reduction of IFN-λR1.

Figure 6

Human DCs were transfected with control siRNA (siCtl) or three sets of duplexes of IFN-λR1 siRNA for 24 h and then infected with DENV for an additional 48 h. Cells were collected, and the levels of IFN-λR1 protein (A) were determined. In parallel, cells were collected for measurement of chemotactic activity by transwell assays, using CCL19 or CCL21 as a chemoattractant (B). Human DCs transfected with control siRNA (siCtl) or IFN-λR1 siRNA (siIFN-λR1) for 24 h were infected by mock or DENV for an additional 48 h. Cells were collected for measurement of CCR7 mRNA levels by quantitative RT/PCR and cell surface expression by flow cytometry (C). The effects of recombinant IFN-λ1 on DC migration (D) and CCR7 mRNA and cell surface expression (E) were determined. MFI, mean fluorescence intensity. The data show results pooled from at least three independent experiments examining different donor DCs. The analysis was performed by ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Ctl, control.