Figure 2. BSE and SFN prevented diabetes-induced cardiac hypertrophy and glycogen accumulation.

db/db and age-matched WT mice were fed with BSE or SFN every other day for 3 months. After 3 months of treatment, mice were euthanatized, then the ratio of heart weight to tibia length (A) was calculated. Cardiac tissue FITC-conjugated WGA staining (B) and quantification of myocyte cross-sectional areas (C) were analyzed. The expression of cardiac hypertrophic marker and ANP (D) was detected by Western blot. PAS staining (E) was performed to examine glycogen accumulation followed by semi-quantitative analysis of staining. Data are presented as the mean ± S.D. n = 6. *P < 0.05 vs. WT; ^#P < 0.05 vs. db/db.