Figure 4. BSE and SFN increased the expression of Nrf2 and induced Nrf2 nuclear translocation in the heart.

The expression of Nrf2 was detected by Western blot (A). The activation of Nrf2, which was reflected by its nuclear accumulation, was quantified by Western blot (B) of the cardiac nucleus protein. IF staining (C) was also performed to detect the nucleus accumulation of Nrf2 in hearts with Nrf2 antibody in red and nuclear staining by 4,6-diamidino-2-phenylindole (DAPI) in blue; merged blue and red staining indicates nuclear localization of Nrf2. Data are presented as the mean ± S.D. n = 6. *P < 0.05 vs. WT; ^#P < 0.05 vs. db/db.