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. 2016 Jul 26;6:30297. doi: 10.1038/srep30297

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Copyright © 2016, Macmillan Publishers Limited

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A total of 41 homozygous Arabidopsis T-DNA insertion mutant lines corresponding to 26 AtRH genes were selected for TuMV infection assay. The Arabidopsis atrh T-DNA insertion mutants and WT plants were mechanically inoculated with TuMV. ELISA analysis was used to determine the accumulation of TuMV CP in atrh T-DNA mutants and WT plants 10 days post inoculation (dpi). Extracts from newly-emerged leaves of TuMV-infected individual plants were subjected to ELISA using TuMV CP-specific antibody. The x-axis represents ELISA values. Error bars represent standard deviation (n ≥ 5). Asterisk indicates significantly different from WT plants (unpaired two-tailed Student’s t test, p < 0.05).