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. 2016 Jul 26;6:30357. doi: 10.1038/srep30357

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Copyright © 2016, The Author(s)

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(A) Surface plasmon resonance analysis of the interaction of each PfRH5 protein variant (v1.0–v5.0) with basigin. (B) Capture ELISA using a panel of PfRH5-specific mAbs. Each mAb (2AC7, 4BA7 and QA5) was coated to the plate at a concentration of 5 μg/mL, and each PfRH5 protein variant was tested for binding using a dilution series of protein ranging from 800 ng/mL to 12.5 ng/mL. Each sample was tested in triplicate for each concentration. Bars show the median plus range. Results using five other mAbs are shown in Fig. S2. (C) Free sulfhydryl groups were estimated for v1.0 and v2.0 protein variants using Ellman’s reagent in the presence of 6 M guanidine-HCl. Median and replicate results are shown.

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