Table 1. PfRH5 protein purification steps and process yield.

Purification step	Protein recovered (mg)	Step yield (%)	Process yield (%)
1.25 L PfRH5 v1.0 S2 supernatant	37.5	100	100
TFF, buffer exchange, and filtration	30	80	80
Ni-IMAC elution	3.6	12	10
Con A 4B flow through	3.0	83	8
SEC and concentration	1.6	53	4

Representative data for the amount of PfRH5 v1.0 protein recovered at each step of the purification process, along with the step yield and overall process yield. Protein recovery was monitored using an anti-PfRH5 quantification ELISA.