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. 2016 Jul 26;6:30489. doi: 10.1038/srep30489

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) RT-PCR analysis of SOX9/OPN in HCC cell lines (left) and qRT-PCR analysis of OPN in SOX9⁺/SOX9⁻ HLF and PLC/PRF/5 cells (right, Student’s t-test, *P < 0.05). Data are shown as the mean ± SD. (B) Western blot analysis of SOX9 and OPN in SOX9⁺/SOX9⁻ cells with SOX9-gain/loss of function experiments revealed that SOX9 induced OPN expression. (C) Western blotting with OPN-gain/loss of function experiments showed that OPN did not regulate SOX9 expression. (D–F) OPN did not affect the Wnt/beta-catenin activity including nuclear-localization of beta-catenin, cell proliferation, and the expression of cyclin D1 (D,F Student’s t-test, not significant, E; repeated-measures ANOVA, not significant). Scale bar represents 100 μm.