Figure 2. Representative micrograph images of lung sections and the quantitative histological scores, as well as total cell number and eosinophil percentage in BALF.

Lung tissue sections were stained by hematoxylin & eosin (H&E) and Pikosirius-Red as shown in panel a–c, and panel d–f, respectively. Panel a and d, b and e, c and f correspond to lung sections from control, OVA and OVA/BK-1361 (25 μg/g bodyweight) groups, respectively. The bar in panel a–c equals 50 μm, whereas the bar in panel d-f equals 25 μm. The arrows in panel a–c point to perivascular infiltration (PV), peribronchial infiltration (PB), parenchymal infiltration (PA), epithelial damage (ED), respectively. The arrows in panel d–f point to collagen and fibrin deposition in the bronchiole basement membranes. Panel g displays the quantified histological scores considering the extent of PV, PB, PA and ED in the lung tissue sections from different groups of mice, with score 0 indicating no sign of disease, score 5 indicating profound inflammation for each index, thus 20 being the maximum. Panels h and i display the total cell counts number and the percentage of eosinophils in bronchoalveolar lavage fluid (BALF) from different groups of mice respectively. Data in panel g are representative of two independent experiments with n = 7–9 age-matched female mice for each group, and data in panel h and i are representative of n = 5–6 female mice per group. Results are expressed as mean ± SEM. *P < 0.05, **P < 0.005 versus control. ^#P < 0.05, ^##P < 0.005 versus OVA.