Table 2. Oligonucleotide primers used for RT-PCR.
| Sequence (5′-3′) | Length (bp)a | Accession No.b | ||
|---|---|---|---|---|
| IL-4 | FW | CAAACGTCCTCACAGCAACG | 166 | M25892, X05253, NM_021283 |
| RV | AGGCATCGAAAAGCCCGAAA | |||
| IL-5 | FW | CCCTCATCCTCTTCGTTGCAT | 72 | X06270, X06271, NM_010558.1 |
| RV | CCCTTGGCTACACACTGAGTT | |||
| IL-13 | FW | CCAGCCCTCAGCCATGAAATA | 111 | M23504, L13028, NM_008355 |
| RV | CACCTTGAGTGTAACAGGCCA | |||
| CCL5 | FW | CAGCAGCAAGTGCTCCAATC | 238 | NM_013653 |
| RV | CCATTTTCCCAGGACCGAGT | |||
| CCL11 | FW | AACCCAGAGCCTAAGAACTGC | 236 | NM_011330 |
| RV | CTCGTCCCATTGTGTTCCTCA | |||
| CCL22 | FW | GCTCTCGTCCTTCTTGCTGT | 208 | NM_009137 |
| RV | GGATCGGCACAGATATCTCG | |||
| β-actin | FW | AGAGGGAAATCGTGCGTGAC | 138 | X03672, V01217, J00691, M_007393.3 |
| RV | CAATAGTGATGACCTGGCCGT | |||
| GAPDH | FW | TCACCACCATGGAGAAGGC | 168 | M32599, U09964, NM_008084 |
| RV | GCTAAGCAGTTGGTGGTGCA |
The first column lists the tested cytokines including interleukin (IL) 4, 5, 13; chemokine (C-C motif) ligand (CCL) 5, 11, 22; and references of β-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The second column indicates either forward (FW) or reverse (RV) primer. The sequence of each primer, its length and accession number are listed in the 3rd–5th column. The superscript a, and b denotes amplicon length in base pairs, and Genbank accession number of mRNA and corresponding gene (http://www.ncbi.nlm.nih.gov/), respectively.