Figure 6. Mrs2 KD induced increased vulnerability to cellular stress.

(a,b) Comparison of the cell viability under TNF-α/CHX- or H₂O₂-induced stress between in control and in Mrs2 KD cells. In Mrs2 KD cells, cell damage (decrease rate in cell viability) in the presence of H₂O₂ (a) or TNF-α/CHX (b) is more severe than that in control cells (n = 12 for the control and n = 12 for Mrs2 KD samples), indicating Mrs2 KD cells are more vulnerable to cellular stress. (c) Comparison of ATP level between control and Mrs2 KD cells under H₂O₂ condition by metabolome analysis (n = 6 for each sample). (d) Plot of samples under 4 conditions projected onto the first two principal components (PC1 and PC2) identified PCA using all quantified 104 metabolites (n = 6 or 7 for each sample). Each circle indicates a single sample, while the larger ovals represent 95% confidence regions. Each color indicates 4 groups of conditions.