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. 2016 Jul 26;6:30352. doi: 10.1038/srep30352

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Bioassay of 3-oxo-C12-HSL production using the reporter strain CF11. Blue spots indicate that the diffusible signal was detected by the reporter cells. (b) LC-MS analysis of 3-oxo-C12-HSL levels of PA1201-derived strains at 12 and 36 h after inoculation in PPM medium. (c) Bioassay of C4-HSL production using the reporter strain CV026. Purple spots indicate that the diffusible factor was detected by the reporter cells. (d) LC-MS analysis of C4-HSL levels of PA1201-derived strains at 12 and 36 h after inoculation in PPM medium. (e) LC-MS analysis of PQS in PA1201-derived strains at 12 h and 36 h after inoculation in PPM medium. Values shown are the means ± one SD for three independent experiments. Different letters indicate significant differences between treatments at P = 0.05 according to LSD.