Figure 3. Viperin reduced cholesterol and sphingomyelin.

The BSR cells were transfected with pViperin or the pcDNA3.0 plasmid for 24 h, and cholesterol and sphingomyelin contents in BSR cells were measured using kits. Simultaneously, viperin was detected by Western blotting. The data represent averages of three independent experiments. The total amounts of cholesterol and sphingomyelin were determined using colorimetric assays.