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. 2016 Jul 26;6:30529. doi: 10.1038/srep30529

Figure 6. Identification of cellular factors in the TLR4 signal transduction pathway involved in RABV-inducted viperin regulation.

Figure 6

(A) Detection of cellular factors in the TLR4 signal transduction pathway involved in RABV-induced viperin regulation. RAW264.7 cells were infected with rRC-HL at an MOI of 0.1 and were then used to prepare lysates. These lysates were subjected to Western blotting analysis to detect MyD88, IRF3, IFN-β, viperin, NF-κB (p65), RABV N and β-actin protein expression. (B) Based on (A), the supernatants were used to assess IFN-α/β contents using ELISA kits. (C) The RAW264.7 cells were treated with 2,000 pg/mL IFNβ for 24 h, and the cell lysates were then subjected to Western blotting analysis to detect viperin protein expression.