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. 2016 Jun 27;97(3):257–265. doi: 10.1111/iep.12177

Figure 4.

Figure 4

BMI1 is involved in miR‐376c regulation of cervical cancer cell proliferation and invasion. C33A cells were transfected with miR‐376c inhibitor or cotransfected with miR‐376c inhibitor and BMI1‐specific siRNAs(siBMI1), while CasKi cells were transfected with miR‐376c mimics or cotransfected with miR‐376c mimics and BMI1 expression vector(pcBMI1). (a) Western blotting analysis of BMI1 expression in C33A and CasKi cells. (b) Determination of cell proliferation with the MTT assay. (c) Determination of cell invasion ability with the Transwell assay. (d) Flow cytometry analysis of the cell cycle.*P < 0.05, **P < 0.01 compared with control group.