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. 2016 Jul 26;6:30459. doi: 10.1038/srep30459

Figure 2. The increased level of IL-10 receptor1 in cultured primary neurons after OGD injury.

Figure 2

(A) qPCR analysis of IL-10R1 (n = 3). Control group was set as calibrator sample representing the 1 × expression. ***p < 0.001, as compared with the control group; by unpaired two-tailed Student’s t-tests. Data are presented as mean ± SEM. (B) Western blot analysis of IL-10R1 (n = 5). ***p < 0.001, as compared with the control group; by unpaired two-tailed Student’s t-tests. Data are presented as mean ± SEM. (C) Localization of immunoreactive IL-10R1 expression on neurons before and after OGD. The left column displays neuronal marker class III-β-Tubulin (red) and nucleus (blue). The middle column shows expression of IL-10R1 (green), which was expressed in the cyton and neurites. The right column shows the co-localization of IL-10R1 and class III-β-Tubulin (yellow). Scale bar represents 20 μm.