Figure 1. A schematic outline of the HTS approach.

Cells were seeded into a 384-well plate on Day 1, and compounds were added on Day 2. On Day 3, the supernatant from each well was collected. The cells were re-incubated in fresh medium containing MTS reagent for 2 h to assess cell metabolic activity (purple path), while the collected supernatants were assayed for another 24 h (Day 4) for (red path) HTRF-based PGE2 detection.