Table 1. Total protein yields of wild type EGFR-eYFP obtained using the corresponding standard (−) and IRES template (+) in the batch and CECF format.
Fraction | Mode | Time | IRES | Total Protein [μg/ml] |
---|---|---|---|---|
Supernatant fraction | Batch | 2 h | − | 1.8 ± 0.3 |
2 h | + | 5.9 ± 0.4 | ||
CECF | 24 h | − | 8.5 ± 0.4 | |
24 h | + | 57 ± 1 | ||
48 h | + | 43 ± 6 | ||
Microsomal fraction | Batch | 2 h | − | 2.2 ± 0.1 |
2 h | + | 8.5 ± 0.8 | ||
CECF | 24 h | − | 8.4 ± 0.1 | |
24 h | + | 237 ± 6 | ||
48 h | + | 286 ± 21 |
Errors represent the standard deviation of triplicate analysis.