Figure 3. PP2A^Cdc55’s role in monopolar attachment is genetically separable from its role in meiotic FEAR regulation.

(a) spo11Δ spo12Δ cdc55Δ cells carrying plasmids encoding either wild type CDC55 or class B mutant cdc55 alleles (MP1-7) were sporulated. Spore viability was assessed using the ether-killing assay. spo11Δ spo12Δ and spo11Δ spo12Δ mam1Δ strains served as negative and positive controls respectively. (b) spo11Δ spo12Δ cells and spo11Δ spo12Δ strains carrying either mam1Δ or CDC55 or cdc55-T5 or cdc55-MP or cdc55-MP net1-6Cdk were induced to sporulate by transferring them to SPM followed by incubation for 24 h. Spores were harvested and nuclear division was scored by staining with DAPI (N = 100). Spore viability was determined by dissecting dyads onto YPD plates and grown at 30 °C. Spore viability (n = 100) was scored after 3 days.