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. 2016 Jul 26;6:28994. doi: 10.1038/srep28994

Figure 5. Reproducibility of CGH for two technical replicates of MCF-7 cells.

Figure 5

Comparative Genomic Hybridization of two technical replicates of MCF-7 cells from ATCC lot number 59388743 from Johns Hopkins University at passage number 154 (JHU P154, (A) darkblue and darkcyan), and Brown University and at passage number 150 (BU P150, (B) darkolivegreen and darksalmon) versus human female reference DNA. Within (A,B) respectively, only very minor differences can be seen showing very good reproducibility of CGH. Significant genomic differences detected by the Aberration Detection Method 2 (ADM-2) are indicated by the respective horizontal lines at −2 and +2, respectively. All experiments have been performed in one laboratory (JHU) by one individual to exclude any possible inter-laboratory and/or inter-operator effects on reproducibility.