Table 1. STR profiling of genomics MCF-7 DNA directly prepared from two original ATCC vials of the same ATCC batch.
| Loci | ATCC BU | ATCC JHU | Brown P150 | JHU P154 | ATCC reference |
|---|---|---|---|---|---|
| D5S818 | 11,12 | 11,12 | 11,12 | 11,12 | 11,12 |
| D13S317 | 11 | 11 | 11 | 11 | 11 |
| D7S820 | 8,9 | 8,9 | 8,9 | 8,9 | 8,9 |
| D16S539 | 11,12 | 11,12 | 11,12 | 11,12 | 11,12 |
| vWA | 14,15 | 14,15 | 14,15 | 14,15 | 14,15 |
| TH01 | 6 | 6 | 6 | 6 | 6 |
| AMEL | X | X | X | X | X |
| TPOX | 9,12 | 9,12 | 9,12 | 9,12 | 9,12 |
| CSF1PO | 10 | 10 | 10 | 10 | 10 |
STR profiling of MCF-7 cells from two separate shipments but the same ATCC lot number (ATCC #HTB-22, lot number 59388743, passage number 147). ATCC BU and ATCC JHU are the STR profiles from the original shipped vials whereas BU P150 and JHU P154 are the respective STR profiles from passage 150 and 154. All four samples and the reference typing from ATCC show the same STR profiles. All experiments have been performed in one laboratory (JHU) by one individual to exclude any possible inter-laboratory and/or inter-operator effects on reproducibility.