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. 2016 Jul 26;18:71. doi: 10.1186/s13058-016-0729-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Quantification of cell proliferation and apoptosis in mammary gland and tumors. a Photomicrograph (×20 original magnification) of Ki-67 immunostaining (cells indicated by arrows). b Quantification of cell proliferation in the mammary gland ducts and lobules of 50-day-old female offspring of control diet (CO)-, lard (LB)-, and corn oil (CB)-fed male rats. c Quantification of cell proliferation in mammary tumors of female offspring from CO-, LB-, and CB-fed male rats. d Photomicrograph (×40 original magnification) showing apoptotic cells (indicated by arrows). e Quantification of apoptosis in the mammary gland ducts and lobules of 50-day-old female offspring of CO-, LB-, and CB-fed male rats. f Quantification of apoptosis in mammary tumors of female offspring of CO-, LB-, and CB-fed males. Statistically significant difference (p ≤ 0.05) compared with ^aCO and ^bLB, according to analysis of variance followed by a least significant difference test. Marginal difference (p ≤ 0.06) compared with ^cCO and ^dLB, according to t test. The data are expressed as mean ± SEM (n = 4 per group)