Fig. 3.
GalNAc-containing glycoconjugates are sensitive to phosphatidylinositol-specific phospholipase C (PI-PLC) exposure. Lysate of Lu. longipalpis midguts was fractionated by Triton X-114 and analyzed by SDS-PAGE and western blot with lectin from Helix pomatia (HPA). HPA-binding molecules were detected mostly in the detergent phase (DET PLC-) and partially in the aqueous phase (AQ PLC-). In contrast, the HPA-binding molecule in midgut lysate after exposition to PI-PLC was detected mostly in the aqueous phase (AQ PLC+) and not in the detergent phase (DET PLC+)