Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 25;9:413. doi: 10.1186/s13071-016-1695-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 5 — Recombinant Lulo protein has a molecular weight around 40–45 kDa and is recognized by anti-rLulo antibodies. The recombinant Lulo protein was purified by affinity chromatography with lectin from Helix pomatia (HPA). Eluent together with lysate of Lu. longipalpis midguts was analyzed by SDS-PAGE, followed by western blot with anti-rLuloG antibodies. Antibodies against rLuloG recognized one molecule in fraction eluted with N-acetyl-galactosamine (GalNAc), which has molecular weight around 40–45 kDa (Lane 1). Preimunne serum was used as a control, which did not recognize any molecules in fraction eluted with GalNAc (Lane 1c). The recombinant molecule was labeled also with HPA (Lane 2) and the reaction was inhibited by specific GalNAc (Lane 2c). Anti-rLulo serum recognized also the native molecule in a midgut lysate of Lu. longipalpis (Line 3), preimunne serum did not react with the Lu. longipalpis midgut lysate (Lane 3c)