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. 2016 Jul 25;16:239. doi: 10.1186/s12906-016-1181-2

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Nile red staining of HepG2 Cells and quantitative analysis of lipids. Inhibitory effect of AOE on lipid accumulation in HepG2 cell line. Nile red fluorescence was measured using flow cytometry. Fluorescence dot plot and relative fluorescence graph. Values are mean ± SEM, ^### p < 0.001, compared to control, ^* p < 0.05, compared to NEFA-treated group. AOE, Alisma orientale extract; NEFA, non-esterified fatty acid