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. 2016 Jul 25;16:239. doi: 10.1186/s12906-016-1181-2

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Inflammatory protein expression levels. Inflammatory protein regulatory effect of AOE in HepG2 cells. Cell lysates were obtained using NP40 lysis buffer and 15 μg of protein was loaded on a 12 % SDS-PAGE gel. After separation, proteins were transferred to a PVDF membrane. Protein blot was detected using a Davinci-K chemiluminescence detector. β-actin protein was used as an internal control. AOE, Alisma orientale extract; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis