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. 2016 Jul 25;17:158. doi: 10.1186/s13059-016-1017-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Senescence-associated heterochromatic foci (SAHF) are enriched for H4K20me3. a Immunofluorescent images of control (CON) and OIS cells co-stained with antibodies against H4K20me3 and PML. b Cells from a stained with antibodies to H4K20me3 and 53BP1. c Cells from a stained with antibodies to H4K20me3 and γ-H2AX. d Cells from a co-stained with 4′,6-diamidino-2-phenylindole (DAPI) and an antibody against H4K20me3. e Linescan intensity analysis of H4K20me3 and DAPI fluorescence intensity profiles along the arrows indicated in d. f Cells from a stained with antibodies to H4K20me3 and H3K9me3 and DAPI. g Linescan intensity analysis of H4K20me3 and H3K9me3 and DAPI fluorescence intensity profiles along the arrow indicated in f