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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Pain. 2016 Aug;157(8):1744–1760. doi: 10.1097/j.pain.0000000000000578

Fig. 5. Facial formalin injection induces FOS expression in inhibitory neurons in TCC.

Fig. 5

(A) Representative images of FOS-positive cells and GFP-positive cells in caudal Vc of heterozygous GAD67-GFP mice that received saline or 1% formalin injection into the periorbital skin. Arrows indicate FOS-ir in GFP-positive cells.

(B) Total duration of nocifensive behavior in response to intradermal injection of 20 µl saline or 1% dilute formalin at the periorbital region of heterozygous GAD67-GFP mice. The 1st and 2nd phases include behavior 0–10 min and 10–60 min after injection, respectively (n = 3 mice in each group; *p < 0.05, **p < 0.01, two-tailed t-test, compared with the corresponding saline group).

(C) Average number of FOS-positive TCC neurons in heterozygous GAD67-GFP mice that received periorbital injection of saline and formalin, respectively (same mice as in B; ***p < 0.001, two-tailed t-test).

(D) The percentage of FOS-positive neurons that are GFP-positive (same mice as in B; ***p < 0.001, two-tailed t-test).