FIG. 1.

Benzo[a]pyrene (BaP) induces germ cell loss in mouse fetal ovaries. Total number of germ cells was counted as described in Materials and Methods section in 13.5 dpc ovaries cultured for 0, 6, 24, or 48 h with 0, 50, 500, or 1000 ng/ml respectively, BaP in 0.005% DMSO. A, Mean ± SEM total number of germ cells per fetal ovary. Representative images of germ cells in control (B) and BaP-treated (C) ovaries at 48 h identified by TRA98 immunofluorescence. *P = .025, effect of concentration by linear regression. n = 3 − 5/group. Scale bars, 100 µm. UC, uncultured (0 h).