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. 2016 May 13;152(2):372–381. doi: 10.1093/toxsci/kfw083

FIG. 4.

FIG. 4

Benzo[a]pyrene (BaP) does not affect germ cell proliferation in mouse fetal ovaries. Germ cell proliferation was assessed by Ki67 immunostaing in the same ovaries as for Figure 3. A, Mean ± SEM percentage of Ki67 positive germ cells at 0 and 48 h. Representative images of germ cells identified by Ki67 immunodetection in fetal ovaries: 0 h uncultured (B); 0.005% DMSO (C); z-VAD-fmk (D); 1000 ng/ml BaP (E); z-VAD-fmk and 1000 ng/ml BaP cotreatment (F). n = 4–5/group. Ki67 positive cells are dark brown in online version. Arrows point to examples of meiotic germ cells typical of the leptotene stage.