FIG. 5.

Benzo[a]pyrene (BaP) does not deplete germ cells in cultured 13.5 dpc mouse fetal testes. A, Germ cells were counted as described in Materials and Methods section in 13.5 dpc testes cultured for 0, 6, 24, or 48 h with 0, 50, 500, or 1000 ng/ml, respectively, BaP in 0.005% DMSO. Mean ± SEM total number of germ cells identified by TRA98 immunodetection. B, Mean ± SEM percentage of apoptotic germ cells identified by immunohistochemical staining of cleaved caspase-3. n = 3–5/group. UC, uncultured (0 h).