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Upregulation of P53INP1 in response to hydroxyurea treatment. A, Representative blot of P53INP1 protein expression levels in embryos exposed to hydroxyurea compared with controls. B, Quantification of immunoblots showed that hydroxyurea treatment significantly upregulated P53INP1 in the HU400 (FC = 9.95, SEM = ±0.75) and HU600 (FC = 10.78, SEM = 2.29) groups. N = 4–5 for each treatment group. ****P < .0001, one-way ANOVA followed by a Bonferroni post-hoc test.
