Skip to main content
. Author manuscript; available in PMC: 2017 May 15.
Published in final edited form as: Cancer Res. 2016 Feb 26;76(10):3045–3056. doi: 10.1158/0008-5472.CAN-15-2310

Figure 3. CD73−/− mice exhibit decreased early damage to resident cells but similar leukocyte recruitment in response to ionizing radiation.

Figure 3

C57BL/6 WT and CD73−/− mice received 0 or 15 Gy hemithorax irradiation (A, B) or WTI (C, D). (A) Differences in BALF albumin levels of irradiated WT versus CD73−/− mice (Δ albumin) at 3 weeks post-irradiation by ELISA (n = 4/5). (B) Differences in BALF active caspase 3 of WT versus CD73−/− mice (Δ caspase 3) by luminescence [RLU] at 3 weeks post-irradiation (n = 3/3). (C) Active caspase 3 on paraffin-embedded lung sections (lower panels are a 2× enlargement of middle panels: upper & middle panels: scale bar = 100 µm; lower panels: scale bar = 50 µm). Asterisks depict regions with active caspase 3. (D) Time-course of radiation-induced infiltration of CD45+ cells (%) in WT and CD73−/− mice (n = 10/10/10/10, 10/10/10/10, 12/12/12/11, 6/4/6/5, 10/8/8/9 [WT 0 Gy/WT 15 Gy/CD73−/− 0 Gy/CD73−/− 15 Gy]. Data show means ± SD (A, B) or means ± SEM (D). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 by unpaired two-tailed t-test (A, B) or two-way ANOVA followed by post-hoc Bonferroni test (D).