Figure 2.

Frequency and pulse duration effects on use-dependent activation. Cells transfected with Kv1.2 exhibiting prominent use-dependent activation were subjected to repetitive depolarizing steps from −80 mV to + 60 mV, with variable depolarization or resting intervals. Peak current in each pulse was normalized to the peak current observed during a long pulse to +60 mV. In panel (A), the depolarization duration was altered while maintaining a constant pulse frequency of 10 Hz. In panel (B), the interpulse interval was altered while maintaining a constant 10 ms depolarization duration.