FIGURE 5.

Botrytis infection assays in seedlings with a defective SYP123 function. (A–F) The leaves from Col-0, syp123 mutant, and DN-SYP123 plants were inoculated with a spore suspension of Botrytis cinerea. Leaves were stained with trypan blue at 4 days post inoculation (dpi). Dotted circles indicate the borders of spore inoculation sites in control Col-0 (A,D), syp123 mutant (B,E), and DN-SYP123 (C,F). Stained plant cells surrounding the fungal mycelium (m) indicates dead plant cells (dc). (D–F) Shows a higher magnification of the inset from the superior panel at 4 dpi. (G–I) Production of H₂O₂ is visualized by DAB staining in Botrytis inoculated leaves at 4 dpi. The brown precipitate shows DAB polymerization at the site of H₂O₂ production. Representative leaves detached from inoculated Col-0 (A), syp123, and DN-SYP123 plants are shown. All the experiments were performed in triplicate with similar results.