Fig. S7.

Representative histologies from hematoxylin and eosin-stained lung sections of air-exposed sham control and 28 d CS-exposed guinea pigs against those of guinea pigs exposed to CS for 28 d followed by vitamin C or L-NIL administration for additional 60 d, without CS exposure (A). All animals were kept under suboptimal vitamin C treatment (5 mg/kg body weight⋅animal⁻¹⋅d⁻¹) for the 60-d recuperation period without CS exposure to prevent the onset of scurvy. Sections highlight morphological changes in lung alveolar airspace (10 fields, n = 6) due to such treatment. Such alveolar morphometric changes were expressed in terms of Di (B) and Lm (C) as indicators of lung damage. Data were statistically analyzed by paired Student's t test. Significant differences (*P < 0.05) were observed in comparison with unexposed controls. Data are represented as means ± SD and are representative of three independent experiments done under similar conditions. (Scale bars: 50 µm.)