Table S1.

MDNP delivery of firefly luciferase-encoding mRNA payloads to multiple cell types in culture

		Treatment
Cell type	Species/origin	PBS	MDNP Luc mRNA
HeLa	Human epithelial	162.9 ± 8.1	1,769.3 ± 255.9
HFF	Human primary fibroblast	160.8 ± 15.2	586.0 ± 113.6
C2C12	Mouse skeletal myoblast	51.5 ± 3.6	1,971.6 ± 617.4
L6	Rat skeletal myoblast	16.8 ± 3.6	191.4 ± 26.8
MEF	Mouse primary fibroblast	108.3 ± 27.6	454.3 ± 128.2
DC2.4*	Mouse dendritic cell	16.5 ± 3.6	69.2 ± 14.0

Expression measured by luciferase assay as described in SI Materials and Methods, and reported in RLU ± SD of three biological replicates. HFF, human foreskin fibroblast; MEF, mouse embryonic fibroblast; RLU, relative light unit.

^*No signal observed for unmodified mRNA, so base-modified (5-methyl-cytidine and pseudouridine) mRNA was used.