Fig. 1.

Recovery of TCR pairs by emulsion RT-PCR from 5KC hybridomas. (A) Ten 5KC T-cell hybridomas were mixed in equal numbers, and emulsion RT-PCR was performed either with or without emulsion phase. Sequences were analyzed for pairing with the correct alpha-TCR (black) or beta-TCR (gray). Percentages represent the number of reads that paired with the input alpha or beta out of the total number of reads for the analyzed alpha or beta (n = 5 replicate experiments). (B) Frequency that a given TCR pair was recovered after being added in equivalent numbers is shown as a percentage of the total reads recovered (n = 5 replicate experiments). (C) Real-time quantitative PCR for the transcript abundance in the various 5KC hybridomas before addition to the emulsion RT-PCR normalized to the amount in the 1234 hybridomas (n = 3 replicate experiments). Error bars represent SD from the mean. The average read count was 670,000 across five experiments.