Table S1.

Primers for TRAV gene fragments

Conc, nM	TRAV gene	Complement TRBV primer overlap	TRAV-specific	Real-time PCR Ct*
60	1	attgggcaggttgtccctgatt	AGGAGCTCCAGATGAAAGACTC	29.39
60	2	attgggcaggttgtccctgatt	GCTCATCCTCCAGGTGCGGGAG	17.23
60	3	attgggcaggttgtccctgatt	CCTCCTTCCACCTGAAGAAACC	29.73
60	4	attgggcaggttgtccctgatt	CCTGAGCGACACTGCTGTGTAC	28.94
60	5	attgggcaggttgtccctgatt	CTGTCTCTGCGCATTGCAGACA	18.95
60	6	attgggcaggttgtccctgatt	GACTGAAGGTCACCTTTGATACC	33.36
60	7	attgggcaggttgtccctgatt	GCCGTGCAGCCTGAAGATTCA	33.70
60	8	attgggcaggttgtccctgatt	CCTGTGATTTCCTATGCCTGTC	22.31
60	8-1	attgggcaggttgtccctgatt	TCCTTTAATCTGAGGAAACCCTC	25.56
60	8-2	attgggcaggttgtccctgatt	GAATTTAAGAGGAGTCAATCTTCC	30.28
60	8-3	attgggcaggttgtccctgatt	CATCAGAGGTTTTGAGGCTGAAT	19.41
60	8-4	attgggcaggttgtccctgatt	CATCAACGGTTTTGAGGCTGAAT	16.37
60	9	attgggcaggttgtccctgatt	GAAACCACTTCTTTCCACTTGGA	30.77
60	10	attgggcaggttgtccctgatt	CTGCACATCACAGCCTCCCAG	26.79
60	11	attgggcaggttgtccctgatt	GAATATCGCAGCCTCTCATCTG	29.34
60	12	attgggcaggttgtccctgatt	CAGTGATTCAGCCACCTACCTC	26.95
60	13-1	attgggcaggttgtccctgatt	GACAGCCAAACATTTCTCCCTG	27.38
60	13-2	attgggcaggttgtccctgatt	GACAGTGAAACATCTCTCTCTGC	24.30
60	14	attgggcaggttgtccctgatt	CCAACCTTGTCATCTCCGCTTC	30.14
60	15	attgggcaggttgtccctgatt	GAATATGCTGGTCTCTCATCCTG	29.31
60	16	attgggcaggttgtccctgatt	CCATTTGCTCAAGAGGAAGACTC	30.20
60	17	attgggcaggttgtccctgatt	CTTGTTGATCACGGCTTCCCG	28.25
60	18	attgggcaggttgtccctgatt	AGAAGCCCTCGGTGCAGCTG	17.24
60	19	attgggcaggttgtccctgatt	CCACCAGTTCCTTCAACTTCAC	29.69
60	20	attgggcaggttgtccctgatt	CACATCACAGCCCCTAAACCTG	19.99
60	21	attgggcaggttgtccctgatt	CATCAGGACGTAGTACTTTATACA	32.85
60	22	attgggcaggttgtccctgatt	GTACATTTCCTCTTCCCAGACC	28.35
60	23	attgggcaggttgtccctgatt	GCAGTTCTCATTGCATATCATGG	23.95
60	24	attgggcaggttgtccctgatt	ATCAAAGGATCCCAGCCTGAAG	29.36
60	25	attgggcaggttgtccctgatt	GCACATCACAGCCACCCAGAC	28.69
60	26-1	attgggcaggttgtccctgatt	AGTCCAGCACCTTGATCCTGC	25.82
60	26-2	attgggcaggttgtccctgatt	ACAGAAAGTCCAGTACCTTGATC	30.31
60	27	attgggcaggttgtccctgatt	AGGACAGTTCTCTCCACATCAC	30.18
60	28	attgggcaggttgtccctgatt	CTATACATCAGATTCCCAGCCTG	23.75
60	29	attgggcaggttgtccctgatt	GAGACTCTGCAGTGTACTTCTG	28.51
60	30	attgggcaggttgtccctgatt	GCAAAGCTCCCTGTACCTTACG	32.32
60	31	attgggcaggttgtccctgatt	GCTTATCATATCATCATCACAGCC	28.13
60	32	attgggcaggttgtccctgatt	CCCAACCAGGAGACTCATTCC	31.49
60	33	attgggcaggttgtccctgatt	TCCTTAAAACTGACTCAGCCAAG	29.19
60	34	attgggcaggttgtccctgatt	GCAAAGTTCCCTGCATATCACAG	31.65
60	35	attgggcaggttgtccctgatt	AGCTTCCTGAATATCTCAGCATC	27.92
60	36	attgggcaggttgtccctgatt	CAGCATCCTGAACATCACAGCC	28.48

Conc, concentration; TRAV, TCR variable region alpha.

^*Real-time PCR Ct values were determined using PBL cDNA from four control donors.