Fig. S3.

Overexpression is not entirely accountable for the P481R phenotype. (A) MEF cells were obtained from GR-null mice and immortalized as previously described (3). Next, the established MEF GR knocked-out cell line was transduced with GFP-GR wild type (WT) or P481R. Images show representative cells treated with Dex. (Scale bars: 5 μm.) (B) N&B assay. The figure shows the fold increase in ε relative to the control (n = 29, 51, and 42).