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. 2016 Jun 29;113(29):E4238–E4247. doi: 10.1073/pnas.1603229113

Fig. 2.

Fig. 2.

High-density arrays each containing ∼4,600 unique ORFs spotted in duplicate. (A) AtNAPPA02 high-density array showing DNA staining by PicoGreen. (B) Global protein expression levels generated by the submerge method and detected with an anti-Halo antibody are similar for most ORFs. (C) Protein interaction between VND4 and 3×HA-VND7 as detected by an anti-HA antibody. (D, Top) The sorted histogram of B indicates signal intensity (black), median intensity (red), median intensity 2× (green), and median intensity 0.5× (blue) from all ORF clones on the array. (D, Bottom) Signal quantification of the PPI array (C) is shown. The histogram indicates signal intensity (blue) corresponding to boxed regions on the arrays (C, Right, Top). The signals from candidate interactors are significantly above Halo average signal + 3-SD cutoff (black arrows).