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. 2016 Jun 30;113(29):8314–8319. doi: 10.1073/pnas.1521600113

Fig. S6.

Fig. S6.

Vesicle fusion enhanced by Ca2+. Fluorescence intensity of bulk-vesicle fusion was measured by lipid mixing. Ca2+ treatment in the presence of Syt1 increases fluorescence by vesicle–vesicle interactions, indicating that low concentration of Ca2+ stimulates synaptic exocytosis. The 100 μM Ca2+ condition was chosen for experiments.