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Bulk-vesicle fusion assay. The 5-IP7 exhibited the most potent inhibition of vesicle fusion. Lipid mixing of t and v vesicles was traced in the presence of different IPs (30 μM) in the presence of 100 μM Ca2+. Plot of the normalized fluorescence change at 5 min for different IPs. Error bars represent SD. **P < 0.01 (one-sample t test).
