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Cell culture chip used for the orthogonality test of the single PLAs. (A) Layout of the chip. Cell culture chambers are arranged in matrix configuration, which enables 2D antibody screening. (B) Implementation of the antibody titration experiment on the matrix cell culture chip. hASCs were grown for 24 h before fixation. Next, antibodies with various concentrations were applied. Each row and column had one defined antibody concentration, which is indicated within the plot.
