Fig. 1.

Emphysema-like alveolar phenotype in Pofut1^cNull mice. H&E staining of controls (A, C, and E) and Pofut1^cNull (B, D, and F) at different ages. (A and B) No difference at E18.5. (C and D) Marked decrease in the secondary septa (arrowhead) in Pofut1^cNull mice compared with controls at P3. (E and F) At P21, Pofut1^cNull showed enlarged and simplified alveoli. (G) Significantly increased chord length in Pofut1^cNull mice at P21. (H) Pro-SPC–positive type II cells were significantly reduced at P21 in Pofut1^cNull lungs. The cell numbers were counted on 10 fields at 20× magnification of three mice for each genotype. Cleaved caspase-3 showed no difference between wild-type (I) and Pofut1^cNull lungs (J) at P3. Immunohistochemistry of Ki67 and SPC in control (K) and Pofut1^cNull lungs (L) revealed proliferation index significantly decreased in type II cells at P3 (M) (n > 4 in each group; *P < 0.05). (Scale bars, 50 μm in A–F and 10 μm in I–L.)