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Decreased epithelial proliferation in distal bronchioles of Notch2cNull mice. Lung epithelial proliferation was assayed by E-Cad and pHH3 (A and B) or Ki67 (D and E) double staining in control and Notch2cNull lungs at P4. (C and F) Number of E-Cad/pHH3 and E-Cad/ Ki67 double-positive cells in 30 photos of the distal bronchioles (n = 3 in each group). (Scale bars, 50 μm in A, B, D, and E.)
