Table 3.
List of cancer cells that have been cultured in 3D systems.
| Tumor type | Cell Line | Biomaterial | Applications | Refrence |
|---|---|---|---|---|
| Breast Cancer | BT-20, MCF-7 | Polydimethylsiloxane (PDMS) | Findings suggested that the 3D spheroids are more resistant than 2D cultured cells to TRAIL mediated apoptosis and have stem like characteristics (CD44hi, CD24lo, ALDHhi caused due to activation of COX-2/PGE-2 signaling pathways. | Chandrasekaran, PlosOne, Oct 2014, volume 9, Issue 10 |
| 25 cell lines of Luminal, Basal A and B subtyes. | Matrigel | Comparative analysis of gene expression and signaling was performed between 25 B C cells grown in 2D and 3D cultures. | Paraic A Kenny, Molecular Oncology, 2007 | |
| Lung cancer | H1437, H356, H2170, A549, Chago Kl, H23, H1703 | Laminin rich ECM | Lung cancer cells grown on laminin rich ECM can be differentiated into different subtypes based on their growth patterns as smooth and branched structures. | Magdelena A cinchona, Intergrated Biol 2012, volume 4, issue 4. |
| Prostate Cancer | RWPE-2 LNCaP |
Polydimethylsiloxane (PDMS) | Findings suggested that the 3D spheroids are more resistant that the 2D cultured cells to chemotherapeutic drugs | Karen F Chambers et al PlosONe, Nov 2014, Volume 9, Issue 11. |
| PC-3, PC-3M, PrCa, MDA-Pca-2b, NCI-H660 | Matrigel | PrCa did not form spheroids whereas, PC-3 and PC-3M form well differentiated spheroids which transform into invasive cells. Targeting PI3K pathway blocked growth of the invasive cells in spheroids. | Harma V. Virtanen, PlosOne, 2010, Volumes, Issues 5. | |
| Colorectal Cancer | SW-480, HT-29, DLD-1, LOVO, CACO-2, CACO-205, COLO-206F, | Laminin rich ECM | The migratory, invasive or proliferative capacity of the cells did not change on forming spheroids. However, the gene expression profile of the spheroids altered significantly compared to 2D cultures. Also inhibition of EGFR is less effective in spheroids. | Anna C Luca, Plos One, 2013, Volume 8, Issue 3. |
| Ovarian cancer | MLS | Agar | Growth and radiation sensitivity was measured in 3D spheroids of l00 uM diameter consisting of 20 cell cluster. | R.M. Sutherland, British Journal of Cancer, 1989 |
| HEYA8, SKOV3, HEY, | Ultra-low attachment plate | Differential viral oncolytic efficacy was measured in 3D models of epithelial ovarian cancer | Trevor G Shepherd, Molecular Therapy-Oncolytics, 2015 |