Figure 3.

Cl-CD95L Is a Chemoattractant for Th17 Cells In Vivo

Mice received a single injection of cl-CD95L (200 ng/animal) or vehicle and were sequentially sampled.

(A) T cell populations were obtained from tissues or peritoneal cavity washes. Cells were then restimulated in the presence of PMA and ionomycin for 4 hr and then analyzed by flow cytometry. Cells were identified as follows; Th1 (CD4⁺IFN-γ⁺), Th17 (CD4⁺IL-17⁺), and Treg (CD4⁺Foxp3⁺). Numbers of infiltrating cells were calculated.

(B) Ratios of Th17/Th1 cells per organ were determined 24 hr after injection. Data represent two independent experiments with six mice/group; means ± SEM are displayed.