Figure 4.

NBLAST Search and Clustering Reveal Kenyon Cell Subtypes

(A) Hierarchical clustering (HC) of KCs (n = 1,664). Bars below the dendrogram indicate the γ (green), α′/β′ (blue), and α/β neurons (magenta); h = 8.9.

(B) Plot of all γ neurons. KC exemplars plotted in gray for context.

(B′) HC of γ neurons (I–III); h = 3. Neuron plots of groups I–III. Lateral oblique and posterior views of neurons and lateral view of slice through horizontal lobe.

(B′′) HC of atypical γ neurons (group II in B′) divided into three groups (a–c). Neuron plots of groups a–c, a, and b and c. Group a corresponds to the γd subtype. See also Figure S3.

(C) Mushroom body neuropil and subregions.

(D) Neuron plot of α/β neurons. KC exemplars plotted in gray for context.

(D′) HC of α/β neurons divided into four groups (1–4); h = 3.64. Neuron plots of groups 1–4, which match neuroblast clones AM, AL, PM, and PL in posterior and lateral oblique views.

(D′′) HC of group 2 divided into three subgroups. Lateral oblique, posterior oblique, and dorsal view of a peduncle slice are shown. Red and blue subgroups match core and surface neurons, respectively; green subgroup corresponds to α/β posterior subtype (α/βp) (see also Figure S3D). AcCa, accessory calyx.

(E) Hierarchical clustering of uPNs (non-DL2s) (n = 214) cut into 35 groups (1–35) at h = 0.725. Dendrogram shows glomerulus for each neuron. Inset shows uPNs colored by dendrogram group. Neurons that innervate each glomerulus are indicated by black rectangles under dendrogram. Neurons originating from ventral neuroblast are indicated as vVA1lm and vDA1. Dendrogram groups correspond to unique neuron types, except for DL1 and DA1 neurons, which are split into two groups (12–13, 15–16, respectively) (red arrowhead), and the outlier neuron VM5v in group 9 (red asterisk).

(F) Neurons for groups 1–5 from (E); antennal lobe in green; lateral horn in purple. See also Figure S4.